Zebrafish Chordin Antibody / Chd / Chrd

Chordin functions by binding directly to BMP ligands (such as Bmp2 and Bmp4), preventing them from interacting with their receptors and thereby inhibiting BMP signaling activity in dorsal regions of the embryo. The zebrafish chordin gene is expressed in the organizer region (shield) during early development, and its activity is essential for the formation of dorsal structures such as the notochord, neural plate, and somites. Loss-of-function mutations in chordin lead to ventralized phenotypes, while overexpression can result in dorsalization, underscoring its importance as a morphogen regulator. Due to its highly conserved function across vertebrates, zebrafish Chordin is widely used in studies of axis specification, morphogen gradients, neural induction, and BMP pathway modulation.

Western Blot: 0.5-1 µg/mL

O57472

Zebrafish

E. coli-derived zebrafish Chordin recombinant protein (amino acids H69-H940) was used as the immunogen for the Zebrafish Chordin antibody.

WB

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Chordin antibody is available for research use only.

After reconstitution, the Zebrafish Chordin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Chordin antibody should be determined by the researcher.

Western blot analysis of Chordin protein using Zebrafish Chordin antibody and zebrafish head tissue lysates. Predicted molecular weight ~105 kDa but may be observed at higher molecular weights due to glycosylation.