Zebrafish Ckm Antilichaam / Creatine kinase M / Isoforms a & b

Creatine kinase, muscle also known as CKM is a creatine kinase that in humans is encoded by the CKM gene. This gene is mapped to 19q13.32. The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

A2BHA3

Zebrafish

An E.coli-derived zebrafish Ckma/b recombinant protein (amino acids M1-K196) was used as the immunogen for the Zebrafish Ckm antibody. This antibody will detect the a and b isoforms.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Ckm antibody is available for research use only.

After reconstitution, the Zebrafish Ckm antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Ckm antibody should be determined by the researcher.

IHC staining of FFPE zebrafish spleen tissue with Zebrafish Ckm antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.