Zebrafish Cul1 Antilichaam / Cul1a / Cul1b / Cullin 1

Cul1 functions as a scaffold protein, linking the SKP1 adaptor and the Rbx1 RING-finger protein, enabling the recruitment of specific F-box proteins that confer substrate specificity. Zebrafish cul1 expression is detected during early embryogenesis, particularly in proliferative tissues, reflecting its essential role in controlling cell division and differentiation during development. Functional studies in zebrafish have shown that loss of Cul1 activity can disrupt cell cycle control, leading to developmental defects and altered cell proliferation. Its evolutionary conservation and essential cellular functions make zebrafish Cul1 a valuable model for studying ubiquitin-mediated regulation in vertebrate development and disease models.

Western blot: 0.5-1 µg/mL

Q6NYS3, Q802D4

Zebrafish

An E.coli-derived zebrafish Cul1a/b recombinant protein (amino acids K436-A777) was used as the immunogen for the Zebrafish Cul1 antibody. This antibody will detect the a and b isoforms of Cul1.

WB

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Cul1 antibody is available for research use only.

After reconstitution, the Zebrafish Cul1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Cul1 antibody should be determined by the researcher.

Western blot analysis of Zebrafish Cul1a/b protein using Zebrafish Cul1 antibody with 1) zebrafish head and 2) zebrafish embryo tissue lysate. Predicted molecular weight ~90 kDa.