Zebrafish Estrogen receptor 2b Antilichaam / Esr2b

In zebrafish, Esr2b is expressed in a variety of tissues including the brain, liver, gonads, and developing embryos. Upon binding to estrogens such as estradiol, Esr2b translocates to the nucleus where it binds to specific DNA sequences known as estrogen response elements to modulate gene transcription. Esr2b has been shown to influence processes such as sexual differentiation, oocyte maturation, and endocrine signaling. Esr2b is functionally distinct from the closely related Esr2a and Esr1 receptors, and its expression pattern suggests it may have specialized roles in estrogen signaling pathways. Zebrafish provide a powerful model to study Esr2b function in vivo, including the effects of environmental estrogens and endocrine disrupting compounds on gene regulation and reproductive health. Due to its importance in estrogen mediated gene regulation and its responsiveness to hormonal and environmental cues, zebrafish Esr2b is widely used in research focused on reproductive biology, developmental endocrinology, toxicology, and hormone responsive gene networks.

Western Blot: 0.5-1 µg/mL

B8JJL3

Zebrafish

E. coli-derived zebrafish Estrogen receptor 2b recombinant protein (amino acids R27-N592) was used as the immunogen for the Zebrafish Estrogen receptor 2b antibody.

WB

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Estrogen receptor 2b antibody is available for research use only.

After reconstitution, the Zebrafish Estrogen receptor 2b antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Estrogen receptor 2b antibody should be determined by the researcher.

Western blot analysis of Estrogen receptor 2b protein using Zebrafish Estrogen receptor 2b antibody and 1) zebrafish head, 2) whole female zebrafish, 3) whole male zebrafish and 4) zebrafish embryo tissue lysate. Predicted molecular weight ~66 kDa.