Zebrafish Etf1 Antibody / Etf1a / Etf1b / Eukaryotic peptide chain release factor subunit 1

Eukaryotic translation termination factor 1 (eRF1), also known asTB3-1, is a protein that in humans is encoded by the ETF1 gene. It is mapped to 5q31.2. This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD) . Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6,7, and X.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

A8KB65

Zebrafish

An E.coli-derived zebrafish Etf1a/b recombinant protein (amino acids D9-K344) was used as the immunogen for the Zebrafish Etf1 antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Etf1 antibody is available for research use only.

After reconstitution, the Zebrafish Etf1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Etf1 antibody should be determined by the researcher.

Cytoplasm

Immunohistochemical analysis of Etf1a/b protein using Zebrafish Etf1 antibody and paraffin-embedded zebrafish brain tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.