Zebrafish Etv2 Antibody / ETS variant transcription factor 2 / Etstrp

Etv2 is expressed early in the lateral plate mesoderm and is one of the first transcription factors to mark cells destined to become vascular endothelial cells and hematopoietic progenitors. It activates a network of downstream genes required for the formation of blood vessels and blood cells, including regulators of endothelial identity and vascular integrity. Etv2 also works in coordination with other transcription factors to initiate and stabilize lineage commitment. Loss of Etv2 function in zebrafish results in the absence of major blood vessels and blood cells, underscoring its essential role in mesodermal patterning. Because of its early and transient expression during development, zebrafish Etv2 is a valuable tool for studying the genetic control of vasculogenesis, hematopoiesis, and mesodermal differentiation. Etv2 is also widely used in regenerative medicine and stem cell research as a reprogramming factor capable of inducing endothelial identity. Its functional conservation across species makes zebrafish an ideal model for investigating cardiovascular and blood development, as well as for screening drugs that target vascular and hematopoietic pathways.

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

A0A2R8Q5A3

Zebrafish

E. coli-derived zebrafish Etv2 recombinant protein (amino acids M1-I218) was used as the immunogen for the Zebrafish Etv2 antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Etv2 antibody is available for research use only.

After reconstitution, the Zebrafish Etv2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Etv2 antibody should be determined by the researcher.

IHC staining of FFPE zebrafish kidney tissue with Etv2 antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.