Zebrafish Gnsa Antibody / N-acetylglucosamine-6-sulfatase

N-acetylglucosamine-6-sulfatase, also known as glucosamine (N-acetyl) -6-sulfatase, is an enzyme that in humans is encoded by the GNS gene. The product of this gene is a lysosomal enzyme found in all cells. It is involved in the catabolism of heparin, heparan sulphate, and keratan sulphate. Deficiency of this enzyme results in the accumulation of undegraded substrate and the lysosomal storage disorder mucopolysaccharidosis type IIID (Sanfilippo D syndrome) . Mucopolysaccharidosis type IIID is the least common of the four subtypes of Sanfilippo syndrome.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Q4V902

Zebrafish

An E.coli-derived zebrafish Gnsa recombinant protein (amino acids W224-R341) was used as the immunogen for the Zebrafish Gnsa antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Gnsa antibody is available for research use only.

After reconstitution, the Zebrafish Gnsa antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Gnsa antibody should be determined by the researcher.

IHC staining of FFPE zebrafish pancreas tissue with Zebrafish Gnsa antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.