Zebrafish Il-1 beta Antilichaam / Il1b / Interleukine 1

Il1b is primarily produced by macrophages and other immune cells following recognition of pathogens or cellular damage. Once activated, Il1b initiates a cascade of signaling events that result in the expression of additional cytokines, chemokines, and adhesion molecules, thereby amplifying the inflammatory response. In zebrafish, Il1b is expressed early during immune challenge and is a key marker of immune system activation. Il1b is also involved in the regulation of hematopoiesis, fever, and wound healing. Its expression is tightly controlled at both the transcriptional and posttranslational levels to ensure a balanced immune response. Disruption or overactivation of Il1b can lead to excessive inflammation and tissue damage. Due to its evolutionary conservation and well defined function, zebrafish Il1b is widely used in studies of inflammation, infection, immune system development, and inflammatory disease models. It serves as a valuable tool for drug screening and for investigating the molecular pathways that regulate cytokine signaling in vertebrates.

Immunohistochemistry (FFPE) : 2-5 µg/mL

A0A8M1PI54

Zebrafish

E. coli-derived zebrafish Il-1 beta recombinant protein (amino acids M1-I284) was used as the immunogen for the Zebrafish Il-1 beta antibody.

IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Il-1 beta antibody is available for research use only.

After reconstitution, the Zebrafish Il-1 beta antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Il-1 beta antibody should be determined by the researcher.

Cytoplasm

IHC staining of zebrafish Il-1 beta protein using Zebrafish Il-1 beta antibody, HRP-labeled secondary and DAB substrate. Il-1 beta was detected in a paraffin-embedded section of zebrafish cerebellum tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.