Zebrafish Leo1 Antilichaam / RNA polymerase-geassocieerd eiwit LEO1

The Leo1 protein interacts with RNA polymerase two and other components of the transcription machinery to facilitate proper transcriptional progression and co transcriptional histone modifications. It is especially important in regulating genes involved in developmental pathways and stem cell maintenance. In zebrafish embryos, Leo1 is expressed in proliferative regions such as the developing brain, spinal cord, and somites. Functional studies have shown that disruption of Leo1 leads to defects in neural development, patterning of body structures, and cell cycle progression. These findings highlight its essential role in coordinating transcriptional programs during embryogenesis. Because of its conservation across vertebrate species and its involvement in fundamental gene regulatory processes, zebrafish Leo1 is widely used in studies of transcriptional control, developmental biology, and epigenetic regulation. It is also a useful target for exploring how dysregulation of transcription elongation may contribute to developmental disorders and diseases such as cancer.

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

Q6NYV9

Zebrafish

E. coli-derived zebrafish Leo1 recombinant protein (amino acids E333-K571) was used as the immunogen for the Zebrafish Leo1 antibody.

WB, IHC-P, IF

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Leo1 antibody is available for research use only.

After reconstitution, the Zebrafish Leo1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Leo1 antibody should be determined by the researcher.

Nuclear

Immunofluorescent staining of Leo1 protein using Zebrafish Leo1 antibody (green) and zebrafish embryo tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.