Zebrafish Lft1 Antilichaam / Lefty

In zebrafish embryos, Lft1 is expressed asymmetrically, typically on the left side of the lateral plate mesoderm and near the embryonic midline. Its main role is to inhibit nodal signals from spreading to the right side of the embryo, thereby ensuring that organs such as the heart, liver, and pancreas develop in their correct anatomical positions. Lft1 acts as a feedback inhibitor, maintaining a precise boundary of nodal activity and contributing to the robustness of left right patterning. Disruption of Lft1 expression or function can result in abnormal or randomized organ laterality, making it a critical component of the left right determination network. Zebrafish Lft1 is also involved in regulating mesoderm formation and is influenced by other early patterning cues such as those from the organizer region. Due to its conserved role across vertebrates, zebrafish Lft1 is widely used in studies of embryonic asymmetry, developmental signaling, congenital heart defects, and the genetic regulation of organ positioning.

Western Blot: 0.5-1 µg/mL

Q9PW55

Zebrafish

E. coli-derived zebrafish Lft1 recombinant protein (amino acids R19-K312) was used as the immunogen for the Zebrafish Lft1 antibody.

WB

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Lft1 antibody is available for research use only.

After reconstitution, the Zebrafish Lft1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Lft1 antibody should be determined by the researcher.

Western blot analysis of LFT1 protein using Zebrafish LFT1 antibody and 1) zebrafish head, 2) whole female zebrafish and 3) whole male zebrafish tissue lysate. Predicted molecular weight ~41 kDa.