Zebrafish Lims1 Antilichaam / LIM domein-bevattend eiwit

LIM and senescent cell antigen-like-containing domain protein 1 is a protein that in humans is encoded by the LIMS1 gene. The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

A0A8M9QHP1

Zebrafish

An E.coli-derived zebrafish Lims1 recombinant protein (amino acids E22-A183) was used as the immunogen for the Zebrafish Lims1 antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Lims1 antibody is available for research use only.

After reconstitution, the Zebrafish Lims1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Lims1 antibody should be determined by the researcher.

Immunohistochemical analysis of Lims1 protein using Zebrafish Lims1 antibody and paraffin-embedded zebrafish heart tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.