Zebrafish Luteinizing hormoon beta Antilichaam / Lhb

Zebrafish Lhb is produced by gonadotrope cells in the anterior pituitary and is essential for triggering final oocyte maturation and ovulation in females, as well as steroidogenesis and spermatogenesis in males. Its expression is tightly regulated by gonadotropin-releasing hormone (GnRH) and modulated by sex steroids through feedback mechanisms. The zebrafish model has been instrumental in dissecting the molecular and developmental regulation of lhb expression. Notably, Lhb mRNA becomes detectable during larval stages and increases during sexual maturation. Functional studies using gene knockout and reporter lines have demonstrated that Lhb is indispensable for fertility, and its disruption results in reproductive failure. Due to its conserved structure and function, zebrafish Lhb serves as a valuable model for studying reproductive endocrinology, pituitary cell differentiation, and hormone signaling pathways in vertebrates.

Immunohistochemistry (FFPE) : 2-5 µg/mL

Q6TCF5

Zebrafish

E. coli-derived zebrafish Luteinizing hormone beta recombinant protein (amino acids L24-Y140) was used as the immunogen for the Zebrafish Luteinizing hormone beta antibody.

IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Luteinizing hormone beta antibody is available for research use only.

After reconstitution, the Zebrafish Luteinizing hormone beta antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Luteinizing hormone beta antibody should be determined by the researcher.

IHC staining of zebrafish Luteinizing hormone beta protein using Zebrafish Luteinizing hormone beta antibody, HRP-labeled secondary and DAB substrate. Luteinizing hormone beta was detected in a paraffin-embedded section of zebrafish hypothalamus tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.