Zebrafish Lysozyme Antilichaam / Lyz

Lysozyme functions by cleaving the glycosidic bonds in peptidoglycan, a major structural component of bacterial cell walls, particularly in gram positive bacteria. This activity leads to bacterial lysis and supports the clearance of pathogens from the host. In zebrafish, lysozyme genes are expressed during embryogenesis and are upregulated in response to infection or immune stimulation, making them important markers of immune activation. Zebrafish lysozyme is highly conserved with its mammalian counterparts and is commonly used in research focused on host pathogen interactions, inflammation, and immune system development. It is also a valuable tool for studying the effects of genetic mutations, environmental exposures, or pharmaceuticals on immune responses in vivo. Because of the transparency of zebrafish embryos and the availability of immune cell specific transgenic lines, lysozyme expression can be visualized and quantified in real time during infection and inflammation models. This makes zebrafish lysozyme a widely used marker in developmental immunology and disease research.

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Q90YS5

Zebrafish

E. coli-derived zebrafish Lysozyme recombinant protein (amino acids K19-A151) was used as the immunogen for the Zebrafish Lysozyme antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Lysozyme antibody is available for research use only.

After reconstitution, the Zebrafish Lysozyme antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Lysozyme antibody should be determined by the researcher.

IHC staining of FFPE zebrafish liver tissue with Zebrafish Lysozyme antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.