Zebrafish Metap2 Antilichaam / Methionine aminopeptidase 2 / Isoforms a & b

Methionine aminopeptidase 2 is an enzyme that in humans is encoded by the METAP2 gene. The protein encoded by this gene is a member of the methionyl aminopeptidase family. The encoded protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent proteins. Increased expression of this gene is associated with various forms of cancer, and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. Inhibitors of this gene have also been shown to be effective for the treatment of obesity. A pseudogene of this gene is located on chromosome 2. Several transcript variants encoding different isoforms have been found for this gene.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

A5WVX8, Q7SXK1

Zebrafish

An E.coli-derived zebrafish Metap2a/b recombinant protein (amino acids E191-R464) was used as the immunogen for the Zebrafish Metap2 antibody. This antibody will detect the a and b isoforms.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Metap2 antibody is available for research use only.

After reconstitution, the Zebrafish Metap2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Metap2 antibody should be determined by the researcher.

Cytoplasm

Immunohistochemical analysis of Metap2a/b protein using Zebrafish Metap2 antibody and paraffin-embedded zebrafish brain tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.