Zebrafish Mib1 Antilichaam / Geestbom 1

Mib1 functions by promoting the ubiquitination and endocytosis of Delta ligands on the surface of signal sending cells. This endocytosis is required for activation of the Notch receptor in neighboring cells. In zebrafish embryos, Mib1 is expressed in regions undergoing active cell differentiation and tissue segmentation. Loss of Mib1 function leads to widespread defects in neurogenesis, somitogenesis, and other Notch dependent processes. Studies using zebrafish mutants have shown that Mib1 is critical for lateral inhibition in the developing nervous system, the formation of the segmented body axis, and the maintenance of progenitor pools in various tissues. Because of its conserved function across vertebrates, zebrafish Mib1 is a widely used model for understanding the molecular mechanisms of Notch signaling and its role in development and disease. Zebrafish Mib1 is especially valuable in genetic and pharmacological screens aimed at identifying modifiers of the Notch pathway and related developmental disorders.

Western Blot: 0.5-1 µg/mL

Q804S5

Zebrafish

E. coli-derived zebrafish Mib1 recombinant protein (amino acids I78-K995) was used as the immunogen for the Zebrafish Mib1 antibody.

WB

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Mib1 antibody is available for research use only.

After reconstitution, the Zebrafish Mib1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Mib1 antibody should be determined by the researcher.

Western blot analysis of Mib1 protein using Zebrafish Mib1 antibody and 1) zebrafish head tissue lysates. Predicted molecular weight ~113 kDa.