Zebrafish Msi1 Antibody / Musashi 1 / Isoforms a & b

RNA-binding protein Musashi homolog 1 is a protein that in humans is encoded by the MSI1 gene. This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.

Western blot: 0.5-1 µg/mL

Q5CZN9, Q5BKV4

Zebrafish

An E.coli-derived zebrafish Msi1a/b recombinant protein (amino acids K23-D56) was used as the immunogen for the Zebrafish Msi1 antibody. This antibody will detect the a and b isoforms.

WB

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Msi1 antibody is available for research use only.

After reconstitution, the Zebrafish Msi1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Msi1 antibody should be determined by the researcher.

Western blot analysis of Msi1a/b protein using Zebrafish Msi1 antibody and zebrafish 1) head and 2) embryo tissue lysate. Predicted molecular weight ~39 kDa.