Zebrafish Myod1 Antilichaam / Myoblast determination protein 1 / Myogene factor 1

Zebrafish Myod1 is the functional ortholog of human MYOD1. Both proteins belong to the basic helix loop helix family of transcription factors and share a high degree of sequence and functional conservation. Like its human counterpart, zebrafish Myod1 binds to E box elements in the promoters of muscle genes to drive their expression. During zebrafish development, Myod1 is expressed in the somites and presomitic mesoderm, where it promotes the formation of fast twitch muscle fibers. It works in concert with other myogenic regulators such as Myf5 and Myogenin to establish the muscle differentiation program. Disruption of Myod1 function in zebrafish leads to defects in muscle formation and delayed differentiation of myoblasts. Because of its conserved role in vertebrate myogenesis, zebrafish Myod1 is widely used as a marker of muscle lineage commitment and as a tool in genetic and chemical screening to study muscle development, regeneration, and disease. It also provides a useful model for understanding the regulation of transcriptional networks in myogenic cells.

Western Blot: 0.5-1 µg/mL

Q90477

Zebrafish

E. coli-derived zebrafish Myod1 recombinant protein (amino acids M1-L275) was used as the immunogen for the Zebrafish Myod1 antibody.

WB

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Myod1 antibody is available for research use only.

After reconstitution, the Zebrafish Myod1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Myod1 antibody should be determined by the researcher.

Western blot analysis of Myod1 protein using Zebrafish Myod1 antibody and 1) zebrafish head, 2) whole female zebrafish, 3) whole male zebrafish tissue lysate. Predicted molecular weight ~31 kDa, commonly observed at 35-45 kDa.