Zebrafish Noto Antibody / Notochord homeobox eiwit / Drijvende kop

Zebrafish Noto is considered the functional ortholog of the human NOTO gene. Both zebrafish and human versions of this gene encode homeodomain containing transcription factors that are critical for notochord morphogenesis and cilia driven fluid flow, which influences organ laterality during development. Despite some sequence divergence between species, the developmental roles of zebrafish Noto and human NOTO are highly conserved. In the zebrafish embryo, Noto is expressed in the dorsal organizer, the notochordal plate, and the developing tailbud. It regulates genes involved in axial mesoderm formation and ciliogenesis, and it interacts with other developmental signals including components of the nodal and hedgehog pathways. Loss of Noto function leads to defects in notochord structure, tail elongation, and laterality, including reversed heart and gut looping. Because of its critical role in vertebrate axis and notochord formation, zebrafish Noto is widely used in developmental biology to study early mesodermal differentiation, organ positioning, and congenital disorders related to axis specification. Its functional conservation with the human NOTO gene also makes it a useful model for investigating the genetic basis of developmental syndromes affecting the spine and body plan.

Western Blot: 0.5-1 µg/mL

Q90461

Zebrafish

E. coli-derived zebrafish Noto recombinant protein (amino acids M1-D241) was used as the immunogen for the Zebrafish Noto antibody.

WB

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Noto antibody is available for research use only.

After reconstitution, the Zebrafish Noto antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Noto antibody should be determined by the researcher.

Western blot analysis of Noto protein using Zebrafish Noto antibody and zebrafish embryo tissue lysates. Predicted molecular weight ~27 kDa.