Zebrafish Opa1 Antilichaam / Dynamine-achtige GTPase Opa1

Dynamin-like 120 kDa protein, mitochondrial is a protein that in humans is encoded by the OPA1 gene. It is mapped to 3q29. This protein regulates mitochondrial fusion and cristae structure in the inner mitochondrial membrane (IMM) and contributes to ATP synthesis and apoptosis. This gene product is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. It is a component of the mitochondrial network. Mutations in this gene have been associated with optic atrophy type 1, which is a dominantly inherited optic neuropathy resulting in progressive loss of visual acuity, leading in many cases to legal blindness. Multiple transcript variants encoding different isoforms have been found for this gene.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Q5U3A7

Zebrafish

An E.coli-derived zebrafish Opa1 recombinant protein (amino acids D302-Q568) was used as the immunogen for the Zebrafish Opa1 antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Opa1 antibody is available for research use only.

After reconstitution, the Zebrafish Opa1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Opa1 antibody should be determined by the researcher.

Cytoplasm, membrane

IHC staining of FFPE zebrafish brain tissue with Zebrafish Opa1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.