Zebrafish Ostf1 Antilichaam / Osteoclast stimulerende factor 1

In zebrafish, Ostf1 is strongly expressed in the gills, kidney, and other osmoregulatory organs, where it contributes to the adaptation of cells to changes in external ionic and osmotic conditions. Zebrafish Ostf1 is an ortholog of the human OSTF1 gene. Both zebrafish and human proteins share conserved structural features, including domains involved in transcriptional regulation and protein interactions. Functionally, Ostf1 acts as an intracellular signaling mediator that is activated in response to hyperosmotic stress. In zebrafish, it has been shown to regulate the expression of ion transporters and channels, including those involved in sodium and chloride balance. It may also contribute to the remodeling of gill epithelium and the function of mitochondrion-rich cells during osmotic adaptation. Ostf1 is also implicated in developmental processes and stress signaling pathways beyond osmoregulation. Because of its conservation and functional importance, zebrafish Ostf1 is widely used in studies of environmental stress physiology, aquaculture research, and basic cell signaling.

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Q6TGW5

Zebrafish

E. coli-derived zebrafish Ostf1 recombinant protein (amino acids R57-K190) was used as the immunogen for the Zebrafish Ostf1 antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Ostf1 antibody is available for research use only.

After reconstitution, the Zebrafish Ostf1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Ostf1 antibody should be determined by the researcher.

Cytoplasm

IHC staining of FFPE zebrafish fin tissue with Ostf1 antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.