Zebrafish Pafah1b1 Antibody / Pafah1b1a / Pafah1b1b / Lissencephaly-1

In zebrafish, Pafah1b1 is an ortholog of the human PAFAH1B1 gene. Both zebrafish and human Pafah1b1 share a high degree of sequence conservation and functional similarity. This conservation makes zebrafish an excellent model for studying the biological functions of Pafah1b1 and its role in various cellular processes. Zebrafish Pafah1b1 is involved in the regulation of inflammatory responses, neurogenesis, and vascular development. Pafah1b1 is primarily expressed in the brain, heart, and blood vessels during zebrafish development. It plays an essential role in the formation and maintenance of the vascular system, where it helps regulate vascular permeability and inflammation. In addition to its role in vascular biology, Pafah1b1 is important for neuronal development, particularly in neurogenesis and synaptic plasticity. The zebrafish model is widely used to study developmental processes such as angiogenesis and neurogenesis, and the study of Pafah1b1 in zebrafish offers valuable insights into its functional roles in these pathways. Its involvement in immune modulation and neurodevelopmental diseases also makes zebrafish Pafah1b1 an important protein for modeling human conditions related to vascular diseases, neurological disorders, and inflammatory responses.

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Q7T394, Q803D2

Zebrafish

E. coli-derived zebrafish Pafah1b1 recombinant protein (amino acids I95-R410) was used as the immunogen for the Zebrafish Pafah1b1 antibody. This antibody will detect the a and b isoforms.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Pafah1b1 antibody is available for research use only.

After reconstitution, the Zebrafish Pafah1b1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Pafah1b1 antibody should be determined by the researcher.

Cytoplasm

IHC staining of FFPE zebrafish brain tissue with Pafah1b1 antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.