Zebrafish Pard3aa Antilichaam / Partitionering defect 3

In zebrafish, Pard3aa is an ortholog of the human PARD3 gene. Both zebrafish and human Pard3aa share high sequence similarity and functional conservation, particularly in the regulation of cell polarity and cellular architecture. The similarities between the zebrafish and human versions of Pard3aa make zebrafish an excellent model for studying the molecular mechanisms of cell polarity, embryonic development, and tissue morphogenesis. Pard3aa plays a vital role in the development of epithelial tissues, including the brain, gut, and heart, where it helps to establish and maintain apical-basal polarity. During embryogenesis, Pard3aa is involved in controlling embryo axis formation, cell migration, and the establishment of cellular asymmetry. It regulates the distribution of key proteins involved in junction formation and membrane trafficking, ensuring proper cell-cell adhesion and tissue integrity. Given its role in developmental biology, cellular architecture, and tissue polarity, zebrafish Pard3aa serves as a useful model for studying fundamental processes like neural development, organogenesis, and cell polarity diseases. It is also an important protein for investigating human conditions related to developmental disorders, epithelial cancers, and neurodegenerative diseases that involve defects in cell polarity and cellular organization.

Immunohistochemistry (FFPE) : 2-5 µg/mL

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Zebrafish

E. coli-derived zebrafish Pard3aa recombinant protein (amino acids M1-Q1303) was used as the immunogen for the Zebrafish Pard3aa antibody.

IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Pard3aa antibody is available for research use only.

After reconstitution, the Zebrafish Pard3aa antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Pard3aa antibody should be determined by the researcher.

Cytoplasm, cell membrane, cell junctions (human similarity)

IHC staining of zebrafish Pard3aa protein using Zebrafish Pard3aa antibody, HRP-labeled secondary and DAB substrate. Pard3aa was detected in a paraffin-embedded section of zebrafish eye tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.