Zebrafish Pax6 Antilichaam / Pax6a / Pax6b

During embryogenesis, zebrafish Pax6 is expressed in the forebrain, optic vesicles, retina, and spinal cord, where it regulates the expression of genes critical for cell fate determination and tissue patterning. Pax6 is a key regulator of eye field specification and is required for the proper formation of structures such as the lens, retina, and optic tectum. In addition, it contributes to pancreatic islet cell development, particularly in the differentiation of insulin-producing Beta-cells. Given its evolutionary conservation and well-characterized functions, zebrafish Pax6 is widely used as a model for studying ocular development, neural differentiation, congenital eye disorders (e.g., aniridia), and transcriptional control of organogenesis.

Western Blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL

P26630

Zebrafish

E. coli-derived zebrafish Pax6 recombinant protein (amino acids M1-M401) was used as the immunogen for the Zebrafish Pax6 antibody. This antibody will detect isoforms a & b.

WB, IF

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Pax6 antibody is available for research use only.

After reconstitution, the Zebrafish Pax6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Pax6 antibody should be determined by the researcher.

Nuclear

Immunofluorescent staining of PAX6 protein using FFPE zebrafish embryo tissue and Zebrafish Pax6 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.