Zebrafish Pgk1 Antilichaam / Fosfoglyceraat kinase 1

PGK1 (Phosphoglycerate Kinase 1), also known as PGKA, is an enzyme that in humans is encoded by the PGK1 gene. The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Q7ZV29

Zebrafish

An E.coli-derived zebrafish Pgk1 recombinant protein (amino acids N138-N337) was used as the immunogen for the Zebrafish Pgk1 antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Pgk1 antibody is available for research use only.

After reconstitution, the Zebrafish Pgk1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Pgk1 antibody should be determined by the researcher.

Cytoplasm

IHC staining of FFPE zebrafish brain tissue with Zebrafish Pgk1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.