Zebrafish Ppar gamma Antilichaam / Pparg

In zebrafish, Pparg is an ortholog of the human PPARG gene. The zebrafish and human proteins share a high degree of sequence conservation, particularly in their DNA binding domain and ligand-binding domain, which are essential for their function as transcription factors. This evolutionary conservation suggests that the role of Pparg in regulating lipid metabolism and adipogenesis is preserved across species, making zebrafish a valuable model for studying the molecular mechanisms of metabolic diseases and obesity. Pparg in zebrafish has isoforms, which may exhibit tissue-specific expression and functional variation. The existence of isoforms allows for regulation of different target genes depending on developmental stages, tissue types, and environmental conditions. These isoforms may also be involved in regulating distinct physiological processes, such as fatty acid metabolism, inflammatory response, and immune cell differentiation. In zebrafish, Pparg is expressed in various tissues, including the adipose tissue, liver, and muscles, as well as in developing embryos. Its expression is tightly regulated to ensure proper energy homeostasis and organ development. Pparg plays an essential role in early development, especially in regulating the formation of adipocytes (fat cells), which are important for long-term energy storage and regulation. Disruption of Pparg function in zebrafish can result in defects in lipid metabolism and adipogenesis, providing a model for studying metabolic disorders.

Western Blot: 0.5-1 µg/mL

A6XMH6

Zebrafish

E. coli-derived zebrafish Ppar gamma recombinant protein (amino acids M1-D512) was used as the immunogen for the Zebrafish Ppar gamma antibody.

WB

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Ppar gamma antibody is available for research use only.

After reconstitution, the Zebrafish Ppar gamma antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Ppar gamma antibody should be determined by the researcher.

Western blot analysis of Ppar gamma protein using Zebrafish Ppar gamma antibody and 1) zebrafish head, 2) whole female zebrafish, 3) whole male zebrafish and 4) zebrafish embryo tissue lysate. Predicted molecular weight ~60 kDa.