Zebrafish Ppp1r12a Antilichaam / Mypt1

Zebrafish Ppp1r12a is the ortholog of the human PPP1R12A gene, which encodes the protein also known as myosin phosphatase target subunit one. This protein directs protein phosphatase one to myosin light chains and other cytoskeletal targets, enabling dynamic control of actomyosin contraction and cell movement. It is involved in signaling pathways that govern smooth muscle relaxation, cell shape changes, and cell migration. During zebrafish development, Ppp1r12a is expressed in tissues undergoing active morphogenetic movements and structural rearrangement, such as the developing somites, neural tube, and heart. It contributes to proper tissue organization by regulating the phosphorylation state of cytoskeletal components and by modulating mechanical tension within cells. Disruption of Ppp1r12a function in zebrafish has been shown to affect cell adhesion, motility, and organ formation, highlighting its importance in developmental processes. Due to its conservation across vertebrates and its central role in regulating phosphatase activity, zebrafish Ppp1r12a is a useful model for studying signal transduction, cytoskeletal regulation, and developmental disorders involving cell morphology and contractility.

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Q6DRG7

Zebrafish

E. coli-derived zebrafish Ppp1r12a recombinant protein (amino acids M1-D40) was used as the immunogen for the Zebrafish Ppp1r12a antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Ppp1r12a antibody is available for research use only.

After reconstitution, the Zebrafish Ppp1r12a antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Ppp1r12a antibody should be determined by the researcher.

Cytoplasm

IHC staining of FFPE zebrafish muscle tissue with Ppp1r12a antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.