Zebrafish Prdx4 Antilichaam / Peroxiredoxin 4

In zebrafish, Prdx4 is an ortholog of the human PRDX4 gene, with both proteins sharing a high degree of sequence conservation in their peroxiredoxin domain, which is essential for their antioxidant activity. This functional conservation suggests that Prdx4 in zebrafish performs similar protective roles as its human counterpart in oxidative stress response and cellular homeostasis. The zebrafish model, therefore, offers an excellent system to study the roles of peroxiredoxins in cell survival, protein quality control, and diseases related to oxidative damage. Prdx4 in zebrafish also has isoforms, which may be generated through alternative splicing. These isoforms can exhibit tissue-specific expression patterns and may have distinct functions depending on the cellular environment or developmental stage. The existence of isoforms allows for more precise regulation of redox balance in response to physiological stress and disease conditions, including conditions involving chronic inflammation or neurodegenerative disorders. Zebrafish Prdx4 is expressed in various tissues, including the liver, brain, muscles, and heart, where it plays an essential role in protecting cells from oxidative damage and maintaining cellular integrity. Its expression is especially important in the endoplasmic reticulum, where it participates in the oxidative folding of proteins. Prdx4 also contributes to the regulation of immune responses by modulating inflammatory signaling pathways, making it essential for maintaining immune homeostasis and protecting tissues from inflammatory damage. Given its role in antioxidant defense, cell survival, and immune regulation, zebrafish Prdx4 is an important protein for studying oxidative stress, neurodegenerative diseases, cardiovascular health, and immune system function. Disruptions in Prdx4 function in zebrafish can lead to increased cellular oxidative damage, impaired protein folding, and inflammatory responses, making it a valuable model for investigating diseases related to oxidative stress and cellular dysfunction.

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

A3KP44

Zebrafish

E. coli-derived zebrafish Prdx4 recombinant protein (amino acids S167-N260) was used as the immunogen for the Zebrafish Prdx4 antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Prdx4 antibody is available for research use only.

After reconstitution, the Zebrafish Prdx4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Prdx4 antibody should be determined by the researcher.

Cytoplasm

IHC staining of FFPE zebrafish liver tissue with Prdx4 antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.