Zebrafish Psma5 Antilichaam / Proteasoom subeenheid alfa type 5

In zebrafish, Psma5 is the ortholog of the human PSMA5 gene. The protein sequence and functional domains are highly conserved between zebrafish and humans, indicating a shared role in proteasome-mediated protein degradation. This evolutionary conservation supports the use of zebrafish as a model organism for studying proteostasis, developmental regulation, and disease mechanisms related to proteasome dysfunction. Zebrafish Psma5 may have multiple isoforms generated through alternative splicing. These isoforms could allow tissue-specific or developmental stage-specific regulation of proteasome activity, although the canonical isoform is primarily involved in the assembly and function of the standard 20S proteasome. During zebrafish embryogenesis, Psma5 is broadly expressed in rapidly proliferating and metabolically active tissues, including the developing brain, eye, musculature, and digestive organs. Its expression reflects the universal need for controlled protein degradation in processes such as cell cycle progression, signal transduction, and removal of damaged or misfolded proteins. In human biology, defects in proteasome components including PSMA5 have been associated with neurodegenerative diseases, cancers, and immune system disorders. Zebrafish Psma5, due to its conserved function, serves as a valuable model for functional studies of the proteasome and for the development of therapeutics targeting the ubiquitin-proteasome system.

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Q6TGV6

Zebrafish

E. coli-derived zebrafish Psma5 recombinant protein (amino acids M1-I241) was used as the immunogen for the Zebrafish Psma5 antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Psma5 antibody is available for research use only.

After reconstitution, the Zebrafish Psma5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Psma5 antibody should be determined by the researcher.

Cytoplasmic, Nuclear

IHC staining of FFPE zebrafish spinal tissue with Psma5 antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.