Zebrafish Psmb1 Antilichaam / Proteasoom subeenheid bèta type 1

In zebrafish, Psmb1 is an ortholog of the human PSMB1 gene. The zebrafish and human proteins are highly conserved in sequence and function, indicating evolutionary preservation of this essential proteasome subunit. This makes zebrafish a suitable model organism for investigating proteasome biology and related human diseases. Zebrafish Psmb1 may have isoforms resulting from alternative splicing, although the predominant isoform is primarily responsible for incorporation into the core proteasome structure. These potential isoforms may contribute to tissue-specific regulation of proteasome function or be expressed under certain developmental or stress conditions. During zebrafish development, Psmb1 is ubiquitously expressed, reflecting the widespread requirement for protein turnover in cellular homeostasis, growth, and differentiation. Expression is especially prominent in tissues with high metabolic activity, such as the brain, eye, muscle, and liver. Disruption of Psmb1 expression or proteasome assembly can impair embryonic development and lead to cellular stress due to the accumulation of damaged or misfolded proteins. In humans, alterations in proteasome function including mutations in PSMB1 have been implicated in disorders such as neurodegeneration, cancer, and autoimmune syndromes. Zebrafish Psmb1 is therefore a valuable research target for understanding the roles of the proteasome in development, disease, and therapeutic intervention strategies that modulate proteasomal activity.

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Q6DRF3

Zebrafish

E. coli-derived zebrafish Psmb1 recombinant protein (amino acids Y18-D237) was used as the immunogen for the Zebrafish Psmb1 antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Psmb1 antibody is available for research use only.

After reconstitution, the Zebrafish Psmb1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Psmb1 antibody should be determined by the researcher.

Cytoplasmic, Nuclear

IHC staining of FFPE zebrafish muscle tissue with Psmb1 antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.