Zebrafish Psmc4 Antilichaam / 26S proteasoom subeenheid 6B

PSMC4 is one of six AAA+ ATPases (Rpt1"“Rpt6) that form a hexameric ring within the base of the 19S proteasome regulatory particle. This ATPase activity is required for substrate unfolding and translocation into the 20S core particle for proteolysis. In zebrafish, psmc4 is expressed ubiquitously, with elevated expression in rapidly dividing and metabolically active tissues, underscoring its involvement in cell cycle regulation, signal transduction, and stress responses. Functional analyses in zebrafish models have demonstrated that disruption of psmc4 leads to proteasome dysfunction, accumulation of ubiquitinated proteins, and developmental abnormalities. Because of its central role in protein degradation, zebrafish PSMC4 is a valuable target for studying proteostasis, neurodegenerative disease mechanisms, and the effects of proteasome inhibitors in vivo.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

B0R1D0

Zebrafish

An E.coli-derived zebrafish Psmc4 recombinant protein (amino acids M1-D410) was used as the immunogen for the Zebrafish Psmc4 antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Psmc4 antibody is available for research use only.

After reconstitution, the Zebrafish Psmc4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Psmc4 antibody should be determined by the researcher.

Nuclear, cytoplasmic

IHC staining of FFPE zebrafish colon tissue with Zebrafish Psmc4 antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.