Zebrafish Psmc5 Antibody / 26S proteasoom subunit 8

In zebrafish, Psmc5 is the ortholog of the human PSMC5 gene, and they share high sequence identity as well as functional conservation. The zebrafish and human proteins possess conserved domains critical for ATP hydrolysis and substrate processing, making zebrafish an excellent model for studying proteasome function. Like its human counterpart, Psmc5 in zebrafish may have isoforms resulting from alternative splicing. These isoforms could exhibit differences in regulatory subunit composition or functional roles within the proteasomal complex, especially in specialized tissues or during developmental stages. Expression of Psmc5 in zebrafish is widespread, with notable presence in actively dividing cells, such as those in the brain, heart, and digestive tissues. This distribution underscores its essential role in development and cellular maintenance. In humans, mutations or dysregulation of PSMC5 have been associated with various diseases, including neurodegenerative disorders and cancers, often due to impaired proteasomal degradation. Zebrafish Psmc5 serves as an ideal model for studying proteasome-related diseases and for screening potential therapeutic agents that target the proteasome machinery.

Immunohistochemistry (FFPE) : 2-5 µg/mL

Q6AZC1

Zebrafish

E. coli-derived zebrafish Psmc5 recombinant protein (amino acids N41-K393) was used as the immunogen for the Zebrafish Psmc5 antibody.

IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Psmc5 antibody is available for research use only.

After reconstitution, the Zebrafish Psmc5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Psmc5 antibody should be determined by the researcher.

Cytoplasmic, Nuclear

IHC staining of zebrafish Psmc5 protein using Zebrafish Psmc5 antibody, HRP-labeled secondary and DAB substrate. Psmc5 was detected in a paraffin-embedded section of zebrafish brain tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.