Zebrafish Psmd14 Antibody / 26S proteasoom niet-ATPase regelgevende subeenheid 14

In zebrafish, Psmd14 is an ortholog of the human PSMD14 gene. Both zebrafish and human Psmd14 share substantial sequence conservation, particularly in their role within the 26S proteasome complex, ensuring functional equivalence in protein degradation. This high degree of similarity makes zebrafish a valuable model organism for studying the molecular mechanisms of protein degradation and its impact on various cellular processes, including development, growth, and disease progression. Psmd14 is expressed in a variety of tissues in zebrafish, including the brain, heart, and muscles, reflecting its importance in general cellular maintenance and metabolic processes. It has a critical role in regulating ubiquitin-mediated proteolysis, which is essential for cell cycle regulation, especially during embryonic development, cell differentiation, and tissue regeneration. Zebrafish Psmd14 has isoforms, which may vary in their expression patterns and functional properties. These isoforms are likely to have tissue-specific roles, particularly in highly dynamic tissues such as neural and muscle tissue. The presence of isoforms suggests a degree of functional specialization and adaptability to specific cellular contexts, allowing Psmd14 to be involved in diverse aspects of protein homeostasis and stress responses.

Western Blot: 0.5-1 µg/mL

A3KNN5

Zebrafish

E. coli-derived zebrafish Psmd14 recombinant protein (amino acids D21-H310) was used as the immunogen for the Zebrafish Psmd14 antibody.

WB

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Psmd14 antibody is available for research use only.

After reconstitution, the Zebrafish Psmd14 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Psmd14 antibody should be determined by the researcher.

Western blot analysis of Psmd14 protein using Zebrafish Psmd14 antibody and 1) zebrafish head, 2) whole female zebrafish, 3) whole male zebrafish and 4) zebrafish embryo tissue lysate. Predicted molecular weight ~35 kDa.