Zebrafish Psmd6 Antilichaam / 26S proteasoom niet-ATPase regelgevende subeenheid 6

In zebrafish, Psmd6 is the ortholog of the human PSMD6 gene, sharing high sequence identity and functional conservation. Both zebrafish and human Psmd6 proteins feature conserved domains that are crucial for their role in proteasome function, allowing for the proper assembly and regulation of the 26S proteasome complex. The similarity between zebrafish Psmd6 and human PSMD6 makes zebrafish an excellent model for studying proteasomal mechanisms and related diseases. Psmd6 in zebrafish may also have isoforms arising from alternative splicing. These isoforms may exhibit variations in their expression patterns or regulatory functions, which could influence proteasomal activity in different tissues or stages of development. The presence of such isoforms allows for specific regulation of proteasomal function under different cellular conditions, such as during cellular stress, development, or differentiation. In zebrafish, Psmd6 is expressed in a variety of tissues, particularly in those with high protein turnover, such as the brain, liver, and kidney. This reflects the protein's essential role in regulating protein degradation and maintaining cellular integrity, particularly in actively dividing cells or tissues under stress. Due to its functional conservation with human PSMD6, Psmd6 in zebrafish provides an ideal model for investigating proteasomal dysfunctions related to various human diseases, including neurodegenerative disorders, cancer, and immune-related conditions. Disruptions in PSMD6 function can lead to the accumulation of damaged or misfolded proteins, contributing to disease pathogenesis. The zebrafish model facilitates the study of these diseases and offers opportunities for therapeutic screening targeting proteasomal pathways.

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Q7ZWE5

Zebrafish

E. coli-derived zebrafish Psmd6 recombinant protein. (amino acids T27-Q378) was used as the immunogen for the Zebrafish Psmd6 antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Psmd6 antibody is available for research use only.

After reconstitution, the Zebrafish Psmd6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Psmd6 antibody should be determined by the researcher.

Nuclear, cytoplasmic, extracellular

IHC staining of FFPE zebrafish brain tissue with Psmd6 antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.