Zebrafish Sf3a1 Antilichaam / Splicing factor 3A subeenheid 1

Zebrafish Sf3a1 is an ortholog of the human SF3A1 protein and shares significant sequence and functional conservation. Like its human counterpart, zebrafish Sf3a1 is required for normal RNA processing and proper regulation of gene expression. Due to the importance of splicing in controlling developmental and cellular processes, Sf3a1 function is essential during embryogenesis and tissue differentiation. Currently, there are no known isoforms of zebrafish Sf3a1 that have been characterized, but as with other splicing factors, alternative splicing may generate additional transcript variants that are yet to be fully studied. Research into zebrafish Sf3a1 offers valuable insights into conserved mechanisms of splicing regulation and its roles in development and disease. Antibodies targeting zebrafish Sf3a1 protein are useful tools for examining spliceosomal assembly, RNA processing, and transcriptional regulation. These antibodies are commonly used in applications such as western blot, immunohistochemistry, immunofluorescence, and ELISA, supporting a broad range of molecular and developmental biology studies.

Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

Q90X41

Zebrafish

E. coli-derived zebrafish Sf3a1 recombinant protein (amino acids K16-P646) was used as the immunogen for the Zebrafish Sf3a1 antibody.

IHC-P, IF

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Sf3a1 antibody is available for research use only.

After reconstitution, the Zebrafish Sf3a1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Sf3a1 antibody should be determined by the researcher.

Nuclear

Immunofluorescent staining of Sf3a1 protein using Zebrafish Sf3a1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.