Zebrafish Sf3b3 Antilichaam / Splicing factor 3b subeenheid 3

Zebrafish Sf3b3 is an ortholog of the human SF3B3 protein, with strong sequence conservation and similar functional roles. Like its human counterpart, zebrafish Sf3b3 is indispensable for the regulation of alternative splicing, which contributes to transcriptome diversity and precise control of protein synthesis. Disruption of Sf3b3 function can have severe effects on embryonic development and cellular homeostasis due to the widespread impact of splicing errors. Currently, no distinct isoforms of zebrafish Sf3b3 have been described, but its role as part of the SF3B complex is well established and highly conserved across vertebrate species. The zebrafish model provides a powerful system to study the function of Sf3b3 during development and to investigate splicing defects that may be linked to human diseases. Antibodies against zebrafish Sf3b3 protein are valuable for studying spliceosome assembly, RNA processing, and transcriptional regulation. These antibodies are commonly applied in techniques such as western blot, immunohistochemistry, immunofluorescence, and ELISA, enabling detailed investigations of splicing factor expression and localization.

Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

Q1LVE8

Zebrafish

E. coli-derived zebrafish Sf3b3 recombinant protein (amino acids R728-E982) was used as the immunogen for the Zebrafish Sf3b3 antibody.

IHC-P, IF

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Sf3b3 antibody is available for research use only.

After reconstitution, the Zebrafish Sf3b3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Sf3b3 antibody should be determined by the researcher.

Nuclear

Immunofluorescent staining of Sf3b3 protein using Zebrafish Sf3b3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.