Zebrafish Shha Antibody / Shh / Sonic Hedgehog

Zebrafish Shha is an ortholog of the human sonic hedgehog (SHH) protein and shares a high level of sequence and functional conservation. Like human SHH, zebrafish Shha undergoes proteolytic processing and lipid modification to produce an active signaling molecule that interacts with the Patched receptor and initiates downstream signaling through the Smoothened and Gli transcription factors. This pathway regulates the expression of numerous target genes that influence cell fate and tissue organization. There are no known isoforms of zebrafish Shha, but zebrafish possess another related hedgehog gene called Shhb, which shares overlapping but distinct roles during development. The combination of Shha and Shhb activity helps orchestrate the complex patterning events required for normal embryogenesis. Antibodies against zebrafish Shha protein are valuable tools for studying hedgehog signaling pathways, developmental biology, and tissue patterning. These antibodies can be applied in techniques such as western blot, immunohistochemistry, immunofluorescence, and ELISA to monitor Shha expression and localization in embryonic tissues.

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Q92008

Zebrafish

E. coli-derived zebrafish Shha recombinant protein (amino acids Q208-H409) was used as the immunogen for the Zebrafish Shha antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Shha antibody is available for research use only.

After reconstitution, the Zebrafish Shha antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Shha antibody should be determined by the researcher.

Cytoplasm (ER, Golgi), cell membrane

IHC staining of FFPE zebrafish brain tissue with Shha antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.