Zebrafish Spi1b Antibody

Zebrafish Spi1b is an ortholog of the human SPI1 protein, also known as PU.1, and shares high sequence and functional conservation. Similar to its human counterpart, Spi1b binds to specific ETS motifs in gene promoters and enhancers to activate or repress target genes. It is particularly important in the early stages of myelopoiesis and is a key regulator of macrophage and neutrophil lineage commitment. The use of a Zebrafish Spi1b antibody allows researchers to examine Spi1b expression and localization in developing blood and immune cells. Such antibodies are essential for applications including western blot, immunohistochemistry, and immunofluorescence, enabling detailed characterization of hematopoietic cell populations. A Zebrafish Spi1b antibody can also be used to investigate transcriptional networks controlling immune development and to study the effects of genetic or chemical perturbations on blood cell formation. There are no known isoforms of zebrafish Spi1b, and its expression is tightly regulated during embryogenesis and hematopoietic stem cell differentiation. Using a high-quality Zebrafish Spi1b antibody, researchers can gain insights into conserved mechanisms of immune regulation that are relevant to both zebrafish and humans.

Western Blot: 0.5-1 µg/mL

A0A8M2BG46

Zebrafish

E. coli-derived zebrafish Spi1b recombinant protein (amino acids M1-M297) was used as the immunogen for the Zebrafish Spi1b antibody.

WB

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Spi1b antibody is available for research use only.

After reconstitution, the Zebrafish Spi1b antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Spi1b antibody should be determined by the researcher.

Western blot analysis of Spi1b protein using Zebrafish Spi1b antibody and 1) zebrafish head, 2) whole female zebrafish, 3) whole male zebrafish and 4) zebrafish embryo tissue lysate. Predicted molecular weight ~35 kDa.