Zebrafish Srebf1 Antilichaam / Sterol regelgevend element-bindend eiwit 1

Zebrafish Srebf1 is an ortholog of the human SREBF1 protein, with strong sequence and functional conservation. Like its human counterpart, zebrafish Srebf1 is synthesized as an inactive precursor bound to the endoplasmic reticulum membrane. Upon sensing low sterol levels, the protein undergoes cleavage and translocation to the nucleus, where it binds sterol regulatory elements in target gene promoters to activate transcription. The use of a Zebrafish Srebf1 antibody is valuable for detecting and analyzing the expression and processing of this protein in zebrafish tissues. Such antibodies are suitable for applications including western blot, immunohistochemistry, and immunofluorescence to study lipid metabolism and related regulatory pathways. A Zebrafish Srebf1 antibody can also be employed to monitor how dietary, genetic, or pharmacological factors influence lipid biosynthesis in zebrafish models. There are currently no known isoforms of zebrafish Srebf1. The high degree of conservation between zebrafish and human Srebf1 makes this protein a relevant model for investigating metabolic disorders, including obesity, fatty liver disease, and cardiovascular disease. Using a high-quality Zebrafish Srebf1 antibody, researchers can explore conserved lipid regulatory mechanisms that have direct human health implications.

Immunohistochemistry (FFPE) : 2-5 µg/mL

A6XLD8

Zebrafish

E. coli-derived zebrafish Srebf1 recombinant protein (amino acids H20-L1096) was used as the immunogen for the Zebrafish Srebf1 antibody.

IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Srebf1 antibody is available for research use only.

After reconstitution, the Zebrafish Srebf1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Srebf1 antibody should be determined by the researcher.

Cytoplasm (ER, Golgi, Vesicles), Nucleus

IHC staining of zebrafish Srebf1 protein using Zebrafish Srebf1 antibody, HRP-labeled secondary and DAB substrate. Srebf1 was detected in a paraffin-embedded section of zebrafish liver tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.