Zebrafish Tgfb1 Antilichaam / Tgfb1a / Tgf beta

Zebrafish TGF-beta proteins belong to the TGF-beta superfamily, which also includes activins, nodal, and bone morphogenetic proteins (BMPs) . These ligands signal through serine/threonine kinase receptors, leading to the phosphorylation of receptor-regulated SMADs (R-Smads), which then form complexes with co-Smad (Smad4) and translocate to the nucleus to regulate gene expression. TGF-beta signaling in zebrafish is critical during embryogenesis and tissue morphogenesis. It plays essential roles in mesoderm formation, left-right axis specification, cardiovascular development, and immune system modulation. Specific zebrafish isoforms, such as tgfb1a, tgfb1b, and tgfb3, exhibit distinct expression patterns and developmental functions. For example, tgfb1b is involved in hematopoiesis and immune regulation, while tgfb3 contributes to craniofacial development and epithelial integrity. Due to the genetic tractability and optical transparency of zebrafish embryos, this model system provides unique advantages for in vivo analysis of TGF-β pathway dynamics. Zebrafish TGF-beta proteins serve as valuable tools for studying developmental biology, regeneration, fibrosis, and disease mechanisms, including cancer and immune dysfunction.

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

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Zebrafish

E. coli-derived zebrafish Tgfb1 recombinant protein (amino acids A266-S377) was used as the immunogen for the Zebrafish Tgfb1 antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Tgfb1 antibody is available for research use only.

After reconstitution, the Zebrafish Tgfb1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Tgfb1 antibody should be determined by the researcher.

IHC staining of zebrafish Tgfb1 protein using Zebrafish Tgfb1 antibody, HRP-labeled secondary and DAB substrate. Tgfb1 was detected in a paraffin-embedded section of zebrafish kidney tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.