Zebrafish Tnfa Antilichaam / Tnf alfa

Zebrafish Tnfa is an ortholog of the human TNF alpha protein and shares structural and functional conservation with its human counterpart. Similar to human TNF alpha, zebrafish Tnfa is synthesized as a membrane bound precursor that can be cleaved into a soluble form, which binds to TNF receptors to initiate signaling. The conserved nature of Tnfa makes zebrafish a valuable model for studying the role of TNF alpha in inflammatory and immune related diseases. There are no confirmed isoforms of zebrafish Tnfa, and its function appears to be largely analogous to the human protein. Research using zebrafish Tnfa provides insights into cytokine signaling, inflammation, immune cell development, and host pathogen interactions. Antibodies against zebrafish Tnfa protein are useful for applications such as western blot, immunohistochemistry, immunofluorescence, ELISA, and flow cytometry. These tools help researchers study the dynamics of inflammatory signaling and the effects of therapeutic interventions targeting the TNF alpha pathway.

Western Blot: 0.5-1 µg/mL

Q08CQ3

Zebrafish

E. coli-derived zebrafish Tnfa recombinant protein (amino acids M1-K226) was used as the immunogen for the Zebrafish Tnfa antibody.

WB

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Tnfa antibody is available for research use only.

After reconstitution, the Zebrafish Tnfa antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Tnfa antibody should be determined by the researcher.

Western blot analysis of Tnfa protein using Zebrafish Tnfa antibody and 1) zebrafish head, 2) whole female zebrafish and 3) whole male zebrafish tissue lysate. Predicted molecular weight ~26 kDa.