Zebrafish Twist Antibody / Twist1a / Twist1b / Twist2

Zebrafish Twist is an ortholog of the human TWIST1 protein, with high sequence conservation and functional similarity. Like its human counterpart, Zebrafish Twist activates target genes that are involved in cell differentiation, migration, and the regulation of cellular plasticity. Mutations or dysregulation of Twist in humans can lead to congenital syndromes, such as Saethre-Chotzen syndrome, which is characterized by craniofacial abnormalities and other developmental defects. Zebrafish Twist exists in multiple isoforms, including Isoform 1a, Isoform 1b, and Isoform 2, each of which may have distinct roles in development. Isoform 1a is typically involved in early developmental processes, such as mesodermal patterning and limb formation, while Isoform 1b and Isoform 2 have been shown to participate in the regulation of more specific aspects of neural crest migration and other specialized cell differentiation pathways. These isoforms provide valuable models for studying the diversity of Twist functions and their roles in development and disease. The use of a Zebrafish Twist antibody is valuable for investigating the expression and activity of Twist isoforms in various developmental contexts. These antibodies are useful for applications such as western blot, immunohistochemistry, immunofluorescence, and ELISA to study the spatial and temporal expression patterns of Twist during zebrafish development. A Zebrafish Twist antibody can help researchers understand the role of Twist in regulating cellular behaviors like migration, differentiation, and morphogenesis. By using a high-quality Zebrafish Twist antibody, scientists can gain insights into the molecular mechanisms that govern development, as well as the effects of mutations or environmental factors on Twist-related signaling pathways.

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Q9PTE3, Q568M7, Q5XJI8

Zebrafish

A synthetic peptide corresponding to a sequence in the middle region of zebrafish TWIST protein was used as the immunogen for the Zebrafish Twist antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Twist antibody is available for research use only.

After reconstitution, the Zebrafish Twist antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Twist antibody should be determined by the researcher.

Nuclear

IHC staining of FFPE zebrafish brain tissue with Twist antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.