Zebrafish Yb-1 Antilichaam / Ybx1 / Y-boxbindend eiwit 1

Biological Function: Yb-1 is essential for zebrafish embryonic development. Knockdown or mutation of Yb-1 results in early embryonic lethality due to impaired morphogenesis, suggesting a key regulatory role in zygotic gene activation and maternal RNA clearance. It is also involved in stress responses, regulation of cell cycle genes, and may contribute to sex determination pathways in zebrafish. Expression Pattern: Yb-1 is maternally deposited and ubiquitously expressed during early embryonic stages. As development progresses, its expression becomes more tissue-specific, particularly enriched in proliferative zones and neural tissues. Orthology and Conservation: Zebrafish Ybx1 is orthologous to human YBX1, sharing high sequence similarity and functional conservation. This makes zebrafish a valuable model for studying YBX1-related roles in development and disease, including cancer biology and RNA regulation.

Western blot: 0.5-1 µg/mL

B5DE31

Zebrafish

An E.coli-derived zebrafish Yb-1 recombinant protein (amino acids R275-D310) was used as the immunogen for the Zebrafish Yb-1 antibody.

WB

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Yb-1 antibody is available for research use only.

After reconstitution, the Zebrafish Yb-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Yb-1 antibody should be determined by the researcher.

Western blot analysis of Yb-1 protein using Zebrafish Yb-1 antibody and 1) whole female zebrafish, 2) whole male zebrafish and 3) zebrafish embryo tissue lysate. Predicted molecular weight ~35 kDa.