Zebravis Bdnf Antilichaam / Hersenafgeleide neurotrofe factor

Expression of Bdnf in zebrafish is observed early in embryogenesis and persists in various regions of the central nervous system, including the forebrain, optic tectum, and spinal cord. Zebrafish Bdnf interacts primarily with the high-affinity receptor TrkB (Ntrk2), activating intracellular signaling pathways such as PI3K-Akt, MAPK/ERK, and PLC-γ, which are essential for neurotrophic support and synaptic function. Due to the genetic tractability and optical transparency of zebrafish embryos, this model organism is widely used to investigate Bdnf-mediated neuronal development, behavior, and regeneration. Studies have also linked altered bdnf expression to stress responses, learning, and memory in zebrafish, highlighting its relevance in neurodevelopmental and neuropsychiatric research.

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

A0A8M1P735

Zebrafish

E. coli-derived zebrafish Bdnf recombinant protein (amino acids A27-R278) was used as the immunogen for the Zebrafish Bdnf antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Bdnf antibody is available for research use only.

After reconstitution, the Zebrafish Bdnf antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Bdnf antibody should be determined by the researcher.

IHC staining of zebrafish Bdnf protein using Zebrafish Bdnf antibody, HRP-labeled secondary and DAB substrate. Bdnf was detected in a paraffin-embedded section of zebrafish spinal cord tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.