Zebravis C9orf72 Antilichaam

C9orf72 (chromosome 9 open reading frame 72) is a protein which in humans is encoded by the gene C9orf72. It is mapped to 9p21.2. The protein encoded by this gene plays an important role in the regulation of endosomal trafficking, and has been shown to interact with Rab proteins that are involved in autophagy and endocytic transport. Expansion of a GGGGCC repeat from 2-22 copies to 700-1600 copies in the intronic sequence between alternate 5' exons in transcripts from this gene is associated with 9p-linked ALS (amyotrophic lateral sclerosis) and FTD (frontotemporal dementia) (PMID: 21944778, 21944779) . Studies suggest that hexanucleotide expansions could result in the selective stabilization of repeat-containing pre-mRNA, and the accumulation of insoluble dipeptide repeat protein aggregates that could be pathogenic in FTD-ALS patients (PMID: 23393093) . Alternative splicing results in multiple transcript variants encoding different isoforms.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Q6TLH8

Zebrafish

An E.coli-derived zebrafish C9orf72 recombinant protein (amino acids R40-E77) was used as the immunogen for the Zebrafish C9orf72 antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish C9orf72 antibody is available for research use only.

After reconstitution, the Zebrafish C9orf72 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish C9orf72 antibody should be determined by the researcher.

Cytoplasm

Immunohistochemical analysis of C9orf72 protein using C9orf72 antibody and paraffin-embedded zebrafish brain tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.