Zebravis E-cadherin Antilichaam / Cdh1 / Cadherine 1

E cadherin is a transmembrane glycoprotein that mediates homophilic cell to cell adhesion by forming adherens junctions between neighboring epithelial cells. It is a critical component of the cadherin catenin complex, which connects the actin cytoskeleton to the plasma membrane, thereby regulating tissue architecture and cell polarity. In zebrafish embryos, E cadherin is expressed from the earliest stages of development and is especially important for coordinating cell movements and maintaining cohesion during morphogenesis. Loss or disruption of E cadherin function leads to severe defects in cell adhesion, tissue separation, and embryonic patterning. Because of its conserved role in epithelial biology, zebrafish E cadherin is widely used in research focused on developmental biology, cancer metastasis, tissue regeneration, and epithelial to mesenchymal transition. It also serves as a valuable marker for epithelial cell identity and cell junction integrity in various model systems.

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Q90Z37

Zebrafish

E. coli-derived zebrafish E-cadherin recombinant protein (amino acids Y23-D864) was used as the immunogen for the Zebrafish E-cadherin antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish E-cadherin antibody is available for research use only.

After reconstitution, the Zebrafish E-cadherin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish E-cadherin antibody should be determined by the researcher.

Cell membrane, cytoplasm

IHC staining of FFPE zebrafish esophageal epithelium tissue with Zebrafish E-cadherin antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.