Zebravis Fhl2 Antilichaam / Fhl2a / Fhl2b

Four and a half LIM domains protein 2, also known as FHL-2, is a protein that in humans is encoded by the FHL2 gene. This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants encoding different isoforms have been identified.

Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

Q6AZB7

Zebrafish

An E.coli-derived zebrafish Fhl2 recombinant protein (amino acids K235-I279) was used as the immunogen for the Zebrafish Fhl2 antibody. This antibody will detect the a and b isoforms.

IHC-P, IF

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Fhl2 antibody is available for research use only.

After reconstitution, the Zebrafish Fhl2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Fhl2 antibody should be determined by the researcher.

Cytoplasmic, Nuclear

Immunohistochemical analysis of FHL2a/b protein using Zebrafish Fhl2 antibody, HRP secondary and DAB substrate with paraffin-embedded zebrafish brain tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.