Zebravis Glud1 Antilichaam / Glud1a / Glud1b / Glutamate dehydrogenase

This gene encodes Glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Q6NZ29

Zebrafish

An E.coli-derived zebrafish Glud1a/b recombinant protein (amino acids S40-A539) was used as the immunogen for the Zebrafish Glud1 antibody. This antibody will detect the a and b isoforms.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Glud1 antibody is available for research use only.

After reconstitution, the Zebrafish Glud1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Glud1 antibody should be determined by the researcher.

Cytoplasm (Mitochondria)

IHC staining of FFPE zebrafish brain tissue with Zebrafish Glud1 antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.