Zebravis Hspa5 Antilichaam / Grp78 / Bip

Hspa5 facilitates proper protein folding, prevents aggregation of misfolded proteins, and targets irreversibly misfolded proteins for degradation. It is also a key regulator of ER homeostasis, acting as a sensor for misfolded proteins and an upstream effector in activating UPR pathways, including PERK, ATF6, and IRE1.< In zebrafish, hspa5 is expressed ubiquitously during early embryogenesis and is upregulated in response to physiological and environmental stressors such as hypoxia, oxidative stress, and toxin exposure. Its expression is especially prominent in secretory tissues and rapidly developing organs such as the liver, pancreas, and brain. Zebrafish Hspa5 serves as a useful marker for ER stress and has been widely used in models of developmental defects, neurodegeneration, metabolic disease, and toxicology screening. Due to its evolutionary conservation and functional parallels to mammalian GRP78, zebrafish Hspa5 is a valuable target for studying cellular stress responses and disease mechanisms in vivo.

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Q7SZD3

Zebrafish

E. coli-derived zebrafish Hspa5 recombinant protein (amino acids D103-L650) was used as the immunogen for the Zebrafish Hspa5 antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Hspa5 antibody is available for research use only.

After reconstitution, the Zebrafish Hspa5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Hspa5 antibody should be determined by the researcher.

Cytoplasm (ER)

IHC staining of FFPE zebrafish liver tissue with Hspa5 antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.