Zebravis Integrine-linked kinase Antilichaam / ILK

ILK, also known as Integrin-linked kinase, is a serine-threonine protein kinase. Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK) interacts with the cytoplasmic domain of beta-1 integrin. This gene was initially described to encode a serine/ threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. Recent results showed that ILK contains 5 ankyrin-like repeats, and that the C-terminal kinase domain is actually a pseudo-kinase with adaptor function.

Western blot: 0.5-1 µg/mL

Q6PHD6

Zebrafish

An E.coli-derived zebrafish Integrin-linked kinase recombinant protein (amino acids M1-H203) was used as the immunogen for the Zebrafish Integrin-linked kinase antibody.

WB

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Integrin-linked kinase antibody is available for research use only.

After reconstitution, the Zebrafish Integrin-linked kinase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Integrin-linked kinase antibody should be determined by the researcher.

Cytoplasm

Western blot analysis of Integrin-linked kinase protein using Integrin-linked kinase antibody and 1) zebrafish head, 2) whole female zebrafish, 3) whole male zebrafish and 4) zebrafish embryo tissue lysate. Expected molecular weight: 51-59 kDa.