Zebravis Nedd8 Antilichaam / Nedd8L

NEDD8 is a protein that in humans is encoded by the NEDD8 gene. Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The most substrates of NEDD8 modification are the Cullin subunits of Cullin-based E3 ubiquitin ligases, which are active only when neddylated. Their NEDDylation is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.

Immunohistochemistry (FFPE) : 2-5 µg/mL

F1QMF9, Q6DGU4

Zebrafish

An E.coli-derived zebrafish Nedd8/8L recombinant protein (amino acids M1-G76) was used as the immunogen for the Zebrafish Nedd8 antibody.

IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Nedd8L antibody is available for research use only.

After reconstitution, the Zebrafish Nedd8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Cytoplasm, Nucleus

Immunohistochemical analysis of Nedd8/8L protein using Nedd8 antibody and paraffin-embedded zebrafish spinal cord tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.